Research Technologist - Flow Cytometry

 
Position: Research Technologist - Flow Cytometry

Job ID: 162826

Location: US-TX-Houston

Department: Immunology General

Talent Area: Clinical Support

Full/Part Time: Full-Time

Regular/Temporary: Regular

Shift: Monday-Friday 8a-5p

About Texas Children's Hospital

Since 1954, Texas Children’s has been leading the charge in patient care, education and research to accelerate health care for children and women around the world. When you love what you do, it truly shows in the smiles of our patient families, employees and our numerous accolades such as being consistently ranked as the best children’s hospital in Texas, and among the top in the nation by U.S.News & World Report as well as recognition from Houston Business Journal as one of this city’s Best Places to Work for ten consecutive years.   Texas Children’s comprehensive health care network includes our primary hospital in the Texas Medical Center with expertise in over 40 pediatric subspecialties; the Jan and Dan Duncan Neurological Research Institute (NRI); the Feigin Center for pediatric research; Texas Children’s Pavilion for Women, a comprehensive obstetrics/gynecology facility focusing on high-risk births; Texas Children’s Hospital West Campus, a community hospital in suburban West Houston; and Texas Children’s Hospital The Woodlands, the first hospital devoted to children’s care for communities north of Houston. We have also created the nation’s first HMO for children, established the largest pediatric primary care network in the country and a global health program that is channeling care to children and women all over the world. Texas Children’s Hospital is also academically affiliated with Baylor College of Medicine, one of the largest, most diverse and successful pediatric programs in the nation. To join our community of 14,000 dedicated team members, visit texaschildrenspeople.org for career opportunities. You can also learn more about our amazing culture at infinitepassion.org. At Texas Children’s Hospital, diversity, equity, and inclusion are at the core of our values and mission for all of our patients and families that we serve. We are committed to providing a diverse and inclusive work environment for all faculty, staff and learners throughout our organization. We believe that each member of our one amazing team provides enrichment that allows us to create a lasting legacy for future employees of this great organization.   Texas Children’s is proud to be an equal opportunity employer. All applicants and employees are considered and evaluated for positions at Texas Children's without regard to mental or physical disability, race, color, religion, gender, national origin, age, genetic information, military or veteran status, sexual orientation, gender identity, marital status or any other protected Federal, State/Province or Local status unrelated to the performance of the work involved.

Summary:

We are searching for a Research Technologist -- someone who works well in a fast-paced setting. Our CAP/CLIA certified Clinical Immunology Laboratory at TCH in the Allergy and Immunology Division that offers high complexity tests for immunodeficiencies (In inborn errors of immunity due to genetic disorders of the immune system), immune depletion or reconstitution monitoring in the context of transplantation (organ and hematopoietic) and autoimmune diseases.
We are seeking a Research Technologist that specialize in flow cytometry, has Proficiency in handling blood specimens, cellular and PBMC processing, cryopreservation, immunoassays and other diagnostic immunology analyses on full time basis.

The successful candidates will work with and under the supervision of the Laboratory Director and laboratory manager and duties will include new assay development, technical laboratory procedures, complex testing, assay validation, data tabulation and statical analysis, overall data management, writing validation reports and standard operating procedures, overall test monitoring, maintenance of equipment, troubleshooting and improving assays, coordinate research efforts and good communication skills. In addition, individual will train new employees as needed, participate in projects and activities to further A&I laboratory research initiatives, provide equipment and supply maintenance, as well as data operations while remaining in compliance with good laboratory practices and legal requirement.

Think you’ve got what it takes?



Responsibilities :

1. Isolates peripheral blood mononuclear cells (PBMCs) from whole blood samples and cryopreserves (cryopreservation is done occasionally).
2. Develops and perform flow cytometry assays and other immunoassays independently using the whole blood samples or cells derived from blood.
3. Develops quantitative and functional immunoassays as needed and understand the interpretations of the assay results.

4. Designs multicolor panels (Antibody Selection and Fluorochrome Selection), validating staining, performing compensation, setting up gating strategies, performing batch analysis to obtain quantitative flow cytometry data using BD Diva software for the characterization of lymphocyte population and subsets

5. Experience with aseptic techniques and cell culture maintenance and knowledge about the cell-based assays and cell culture experiments is required.

6. Develops, optimizes, and validates new assays and develops, drafts and follows the laboratory’s SOPs for the clinical assays according to regulatory requirements.

7. Create and maintains accurate research records and data with experimental plan and data promptly. Tabulate data and perform statistical analysis, understand the results interpretation and then draft validation reports for all new assays.

8. Manages reagent stock and perform inventory and order reagent and supplies.

9. Maintains and set ups instruments, performs instrument quality control and function checks, and all instrument maintenance records.

10. works under pressure and ability to make independent decision as required

11. Possesses the Microsoft Office skills including the ability to successfully use all the features of Microsoft word and excel.

12. Provides guidance and training to the clinical laboratory personnel.

13. Expected to contribute to research publications, presentations, and funding applications.

14. Complies with all governmental, regulatory agencies and the institutional rules, regulation

15. Accomplishes professional growth & other duties as assigned.



Qualifications:

Being fully vaccinated against COVID-19, including any booster dose(s) of the COVID-19 vaccine recommended by the Centers for Disease Control when eligible, is required for all employees at Texas Children’s unless approved for a medical or religious exemption

1. Bachelor’s degree in related field (biological or biomedical sciences even though Immunology is preferred) with five (5) years of related research experience; or master’s degree in related field (biological or biomedical sciences even though Immunology is preferred) A Master’s degree may substitute for two (2) years of the required experience.

2. At least 2-5 years of experience with analytical flow cytometry and FACSDiva software is preferred.

3. Excellent verbal and written communication skills are required. ASCP Specialist in Cytometry accreditation is highly desirable.

4. Aseptic techniques experience and willingness to work flexible and extended hours as necessary is preferred.

5. Ability to work in teams as well as independently without supervision is required.

6. Understands and applies personal responsibility and possesses good interpersonal skills.

7. Understands and complies with the regulatory requirements and possess impeccable record keeping skills,

Physical Requirements:
1. Mostly involves sedentary work, frequently or constantly move, lift, carry light weight objects and at times exerts up to 10 pounds of force.

2. This position may include work involving potentially hazardous chemical, biological or radioactive agents.



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